犏牛体外受精胚胎的发育转录组分析

doi:10.11843/j.issn.0366-6964.2018.01.011

Abstract

Abstract:

The objective of this study was to investigate the regulatory mechanism of the early development of hybrid embryos of the yak (Bos grunniens) in order to provide theoretical basis for improving efficiency of in vitro production (IVP). Total RNA were extracted from embryos that derived from yak oocytes in vitro fertilized with Jersey cattle at 5 stages of 2-cell, 4-cell, 8-cell, morula and blastocyst and then amplified via the Smart-Seq2 method to construct RNA libraries and sequenced using RNA-seq. A total of 47 791 850-63 332 216 Clean Reads for each in vitro stage after filtering the Raw Reads of HiSeq^TM2500 sequence were obtained, of which, 80.00% to 91.13% Reads were covered in the reference genome. Number of expressed genes were greatest at 4-cell stage (15 400) and lowest at blastocyst stage (9 604). When|log₂ ratio| ≥ 1 and Q-value<0.05 were set as thresholds for identifying differentially expressed genes (DEGs), a total of 3 690, 6 332, 8 965 and 10 298 DEGs were identified for 2-cell-4 cell, 4-cell-8-cell, 8-cell-morula and morula-blastocyst, respectively. The GO distributions of the DEGs for 4 stages were classified into 3 categories:biological processes (BP), cellular components (CC), molecular functions (MF) with a total of 67 subcategories.KEGG enrichment analysis of DEGs showed that the major enrichment pathways were Spliceosome, Neuroactive ligand-receptor interaction, Cytokine-cytokine receptor interactions, Ubiquitin mediated proteolysis, RNA transport etc. In conclusion, this was the report to investigate the mechanism that the early development of hybrid in vitro-derived yak embryos using high-throughput sequencing. A number of DEGs and pathways were discovered, which provided valuable information to improve in vitro production technique for yak crossbred embryos.

CLC Number:

S823.8⁺5.2

ZI Xiang-dong, LIU Shuang, XIA Wei, XIONG Xian-rong, HUANG Lin, ZHANG Zheng-fan, LI Zhi-xiong, LI Jian, ZHONG Jin-cheng, WANG Li, ZHU Jiang-jiang. Transcriptome Analysis during Development of Hybrid in vitro-derived Yak Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 92-101.

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Transcriptome Analysis during Development of Hybrid in vitro-derived Yak Embryos

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